Figure 2.

Median-joining networks based on puma ND5 haplotypes. The area of each circle is proportional to the haplotype frequency. The relative frequency of populations in each haplotype is indicated by the proportion of different colors (coded as in Figure 1). A) Analysis of the full data set (669 bp). The arrow indicates the point where the outgroup P. yagouaroundi is connected. B) Analysis of a subset of the nucleotide sites that overlap with the Miracinonyx trumani sequence (286 bp). Arrows indicate the points where the outgroup taxa join the network: P. yagouaroundi (in gray; 32 mutational steps from the connection point), and M. trumani (in black; 19 steps from the connection point).