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Discovery of the sphaerimicins. A) Expression analysis of the putative sphaerimicin biosynthetic genes under different cultivation conditions including a) YMA solid media compared to b) CNZ4, c) AP-1, and d) 172F liquid media. B) HPLC analysis of the extracts obtained from the indicated culture broths. A260: absorbance at 260 nm. C) Structure of 11 and hydrolysis products (15 and 16).
