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. 2013 Dec 26;7(12):e2580. doi: 10.1371/journal.pntd.0002580

Figure 3. Competitive ICB ELISA using two cross-reacting samples.

Figure 3

A. Reactivity (P/N ratio) of a DENV-1 sample. Black columns from left to right: without addition of competitive antigen (no comp.) a strong reaction with DeP1 (P/N ratio 21.1) is seen. Next column: Upon competition with De3 (De3+) the reaction with DeP1 (P/N ratio 22) remains almost unchanged. Next column: autologous competition with De1 (+De1) results in a P/N ratio of <1. Cross-reactivity with DeP3 antigen (next three columns): without competition (no comp.) the P/N ratio is 11.5. With competition using De3 (+De3) and De1 (+De1) all reactivity disappears. B. The same experiment is carried out for the DENV-3 sample (grey columns). Without competition (no comp.) a strong reaction with DeP3 antigen is seen (P/N ratio 18.5). Addition of De1 (+De1) does not change the positive reaction (P/N ratio from 18.3 to 18.2). The strong cross-reactivity with DeP1 (P/N ratio 7.1) without competition (no comp.) disappears both upon addition of De3 (+De3) and of De1 (+De1).