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. 2013 Dec 26;9(12):e1004071. doi: 10.1371/journal.pgen.1004071

Figure 3. Meiotic depletion of Smc5 and Nse4 leads to Spo11-dependent nuclear separation defects in meiosis.

Figure 3

(A) Catalytic-dead Spo11 mutation rescues nuclear separation at anaphase I in the Smc5/6 mutants (n≥100). Bars indicate standard error bars for a proportion. Strains: WT (Y1381), smc5 (Y2705), nse4 (Y2704), smc5 nse4 (Y3185), spo11-Y135F (Y3147), spo11-Y135F smc5 (Y3150), spo11-Y135F nse4 (Y3153), spo11-Y135F smc5 nse4 (Y4202). (B and C) Schematic of sister chromatid segregation at meiosis I in spo11Δ spo13Δ mutants. Dyad formation and viability after 24 hours in sporulation medium of Smc5/6 mutants in conjunction with the spo11Δ spo13Δ bypass. Strains: spo11Δ spo13Δ (Y2816), spo11Δ spo13Δ smc5 (Y2846), and spo11Δ spo13Δ nse4 (Y2848).