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. 2013 Dec 26;9(12):e1004071. doi: 10.1371/journal.pgen.1004071

Figure 5. Aberrant joint molecules accumulate in smc5 and nse4 mutants.

Figure 5

(A) Examples of time courses from 2D gels. Blue lines point at joint molecules formed between homologous chromosomes (interhomolog, IH) and red lines indicate joint molecules composed of sister chromatids (intersister, IS). Strains: WT (Y2976), smc5 (Y1211), nse4 (Y1212). (B) Enlarged dHJ spots from wild type, smc5, and nse4. (C) Smoothed levels of single end invasions (SEIs), multichromatid joint molecules (mcJMs), interhomolog-double Holliday Junctions (IH-dHJs), intersister-double Holliday Junctions (IS-dHJs), IH-dHJ to IS-dHJ (IH∶IS) ratio, and total joint molecules (Total JMs). Cumulative levels of recombination were assessed in the ndt80Δ background (lower panel). Strains: ndt80 (Y3025), ndt80 nse4 (Y3843). (D) Examples of time course analyses of double-strand break and crossover formation. (E) Quantification of DSB, crossover, MI+MII nuclear divisions.