Figure 4. Manipulations of signaling components possibly downstream the dInR affect cell body size of larval ABLKs.

Neurons are visualized with anti-LK. A ABLKs in control flies. B Quantification of cell body sizes of LK neurons in the different experiments. C and D expression of a dominant negative form of PI3K (PI3K-DN) diminishes cell body size and overexpression of wild type PI3K increases it (and leads to dislocation and irregular shapes of cell bodies). E and F Expressing Akt-RNAi and wild type Akt gives phenotypes similar to PI3K manipulations. G and H Overexpression of Rheb drastically increases cell body size, whereas Rheb-RNAi has no significant effect (***p<0.001, n = 8–14 animals for each genotype from 3 crosses; unpaired Student's T-test).