Skip to main content
. 2013 Dec 26;9(12):e1004052. doi: 10.1371/journal.pgen.1004052

Figure 7. Dimm expressing neurons respond to manipulations of the dInR and PI3K.

Figure 7

The inset at the center shows the localization in the CNS of third instar larva of neurons depicted in Fig. 6, 7 and Fig. S11. A Using the c929-Gal4 to drive dInR constructs, and an antiserum to FMRFamide, we monitored the cell body size of the thoracic Tv1 – 3 neurons, known to express Dimm. As seen in the smaller panels the cell bodies are smaller after dInR-RNAi and larger after dInR over expression. B Quantification of Tv1–3 cell body sizes (*p<0.05, ***p<0.001, n = 6–8 animals for each genotype from 3 crosses; unpaired Student's T-test). C and D Also the cell bodies of the insulin producing cells (IPCs) of the larval brain respond significantly to manipulations of the dInR as well as PI3K. A dominant negative form of PI3K (PI3K-DN) was used. D Quantification of cell body sizes of IPCs (*p<0.05, **p<0.01, ***p<0.001, n = 6–16 animals for each genotype from 3 crosses; unpaired Student's T-test). E Overexpression of dInR on IPCs also affect IIS and has systemic effects on growth of the CNS. F Quantification of the dInR mediated growth of the CNS. Only overexpression of dInR produces a significant change of CNS size (***p<0.001; unpaired Student's T-test; n = 10–12 animals for each genotype, from 3 crosses).