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. 2013 Dec 26;9(12):e1004052. doi: 10.1371/journal.pgen.1004052

Figure 8. Dimm-negative neurosecretory cells are not affected by dInR manipulations, but by Rheb and PI3K overexpression.

Figure 8

A Two pairs of larval PTTH neurons displayed with Ptth-Gal4-driven GFP. These lateral neurosecretory cells (LNCs; asterisk) supply axon terminations to the ring gland (RG). B and C Effects of manipulations of dInR, Rheb and PI3K on PTTH neurons of third instar larvae. Neurons are displayed with recombinant PtthGal4;gfp. Rheb (B5) and PI3K (B7) over expression leads to a size increase, whereas dInR and dInR-CA expression and RNAi and dominant negative (DN) manipulations have no significant effects. C Quantification of cell body size reveals that only Rheb and PI3K overexpression increases cell body size (**p<0.01, n = 5–15 animals for each genotype from 3 crosses; unpaired Student's T-test. D In the adult brain the PTTH-expressing LNCs undergo apoptosis and instead a subset of the ellipsoid body (EB) R-neurons display GFP. E Again, only the Rheb over expression leads to increased cell body size in R-neurons (***p<0.001, n = 5–10 animals for each genotype from 3 crosses; unpaired Student's T-test). CA, constitutively active; DN, dominant negative.