Skip to main content
. 2013 Dec 26;8(12):e82696. doi: 10.1371/journal.pone.0082696

Table 1. Primers for PCR used in this study.

Primer name Sequence (5′-3′)
β-actin βactinF- atgatatcgccgcgctcg; βactinR- cgctcggtgaggatcttca
GAPDH GAPDHF1- aaggtcatccatgacaactt; GAPDHR1- ggctgcttttaactgggta
Nectin-1 NectinF1- attacgggaaaaagacacag
Nectin-2 Nectin2F1- tgatacctgtgaccgtctct; Nectin2R1- aggatgaaggccaaggac
Nectin-3 Nectin3F1- agttatggagggtgacttga; Nectin3R1- atggctgctactgtttcatt
Nectin-4 Nectin4F1- aaggatcacccacatcct; Nectin4R1- acataggccacctgctt
Nectin3FX1* Nectin3FX1- gcaaagcacaactttcctc
Nectin3FX2* Nectin3FX2- ggaaaatacatctgcaaagc
Nectin3FX3* Nectin3FX3- atgaaacagtagcagccatt
Nectin3FX4* Nectin3FX4- atggcgcggaccctgcggc
Nectin3FX5* Nectin3FX5-cttcattttgtccatccatt
Nectin3FX6* Nectin3FX6- tggctggaatattctgctat
Nectin3RX Nectin3RX- ctaaacataccactccctcct
Nectin-1Z** NectinF1- attacgggaaaaagacacag; Nectin1ZR- acgaacctgaccgtacaagttcttggtcaccctttct
Nectin-2Z** Nectin2F1- tgatacctgtgaccgtctct; Nectin2ZR- actgaacctgaccgtacaagctcactcggattatatgc
Nectin-3Z** Nectin3F- agttatggagggtgacttga; Nectin3ZR- actgaacctgaccgtacacattctttccccatactgt
Nectin-4Z** Nectin4F1- aaggatcacccacatcct; Nectin4ZR- actgaacctgaccgtacacacttgagcatagctccttc
GADPHZ** GAPDH2F- ctgagtacgtcgtggagtc; GAPDHZR2- actgaacctgaccgtacacagagatgatgacccttttg
β-actinZ** βactinZF- ggacctgactgactacctca; βactinZR- actgaacctgaccgtacaagcttctcctt

Nectin3FX1 to FX6 versus Nectin3RX; **Z primers for Q-PCR.