Table 1. Primers for PCR used in this study.
Primer name | Sequence (5′-3′) |
β-actin | βactinF- atgatatcgccgcgctcg; βactinR- cgctcggtgaggatcttca |
GAPDH | GAPDHF1- aaggtcatccatgacaactt; GAPDHR1- ggctgcttttaactgggta |
Nectin-1 | NectinF1- attacgggaaaaagacacag |
Nectin-2 | Nectin2F1- tgatacctgtgaccgtctct; Nectin2R1- aggatgaaggccaaggac |
Nectin-3 | Nectin3F1- agttatggagggtgacttga; Nectin3R1- atggctgctactgtttcatt |
Nectin-4 | Nectin4F1- aaggatcacccacatcct; Nectin4R1- acataggccacctgctt |
Nectin3FX1* | Nectin3FX1- gcaaagcacaactttcctc |
Nectin3FX2* | Nectin3FX2- ggaaaatacatctgcaaagc |
Nectin3FX3* | Nectin3FX3- atgaaacagtagcagccatt |
Nectin3FX4* | Nectin3FX4- atggcgcggaccctgcggc |
Nectin3FX5* | Nectin3FX5-cttcattttgtccatccatt |
Nectin3FX6* | Nectin3FX6- tggctggaatattctgctat |
Nectin3RX | Nectin3RX- ctaaacataccactccctcct |
Nectin-1Z** | NectinF1- attacgggaaaaagacacag; Nectin1ZR- acgaacctgaccgtacaagttcttggtcaccctttct |
Nectin-2Z** | Nectin2F1- tgatacctgtgaccgtctct; Nectin2ZR- actgaacctgaccgtacaagctcactcggattatatgc |
Nectin-3Z** | Nectin3F- agttatggagggtgacttga; Nectin3ZR- actgaacctgaccgtacacattctttccccatactgt |
Nectin-4Z** | Nectin4F1- aaggatcacccacatcct; Nectin4ZR- actgaacctgaccgtacacacttgagcatagctccttc |
GADPHZ** | GAPDH2F- ctgagtacgtcgtggagtc; GAPDHZR2- actgaacctgaccgtacacagagatgatgacccttttg |
β-actinZ** | βactinZF- ggacctgactgactacctca; βactinZR- actgaacctgaccgtacaagcttctcctt |
Nectin3FX1 to FX6 versus Nectin3RX; **Z primers for Q-PCR.