Fig. 2.

Intracellular localization of GFP-tagged ykt6 constructs in HeLa cells. (A) Domain organization of human Ykt6. (B) The structure of the N-terminal domain of yeast Ykt6 (19). The image was generated from the data (Protein Data Bank ID 1H8M) by using rasmac and photoshop (Adobe Systems, Mountain View, CA) software. (C–E) Localization of GFP-ykt6 in HeLa cells. Cells were transfected with GFP-ykt6 (C), GFP-ykt6-ΔN (amino acids 134–198) (D), and GFP-ykt6-F42E (E) and observed with a confocal microscope. (Bar = 10 μm.)