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The carboxyl-terminal Cys-194 and Cys-195 of Ykt6 are required for the Golgi association of GFP-ykt6-F42E. HeLa cells were transfected with GFP-ykt6-F42E containing double cysteines at the carboxyl terminus (C194C195, wild type) (A), C194C195/CS (B), C194C195/SC (C), and C194C195/SS (D) and visualized with a confocal microscope. (Bar = 10 μm.)
