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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Reproduction. 2013 Jun 14;146(1):10.1530/REP-13-0086. doi: 10.1530/REP-13-0086

Figure 3. Emergence of SPG as a “loose cell” population in cultures of total testis cells.

Figure 3

(A) Bright field image of partially digested seminiferous tubules used to initiate culture. (B) Immunohistochemistry for VASA (purple) and GATA4 after 3 days culture of total testis cells. Note remnants of tubule pieces (dark brown) are firmly attached to substratum. (C) Higher power image of culture shown in (B). The VASA-positive cells are rounded and loosely scattered on top of the dense fibroblast-like lawn. Bars = 100 um.