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The expression of patS depends on the HetR dimer. Total RNA was isolated from the cells of the wild-type strain (circles) and C48 (squares) at the times indicated after nitrogen step-down and used as templates for reverse transcription. The relative amount of the patS mRNA was analyzed by real-time PCR. Each point is an average of four independent values and is normalized against the maximum values of the patS mRNA in the wild-type strain at 24 h.
