Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The PatS pentapeptide inhibits the DNA-binding activity of HetR. EMSA with FhetR-1 was performed as described in Fig. 4. The PatS pentapeptide RGSGR (lanes 2–5) or a mixture of the 3 aa present in the pentapeptide (lane 6) in a same molar ratio as in lane 5 was added to the binding solutions before electrophoresis. The HetR concentration was 1 μM, and the molar ratios of PatS/HetR are shown on top of the gel. Lane 1, no HetR was added to the reaction solution.
