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. 2013 Dec 26;8(12):e83631. doi: 10.1371/journal.pone.0083631

Figure 4. CD4 T cells specific for various antigens detected after mCOL7c-GST immunization.

Figure 4

Draining lymph node cells were harvested 5 weeks after immunization and restimulated with no antigen, OVA, mCOL7c-HIS, GST or mCOL7c-GST, as indicated. T cells specific for the respective antigens were identified by electronic gating on CD4+ cells and the rapid up-regulation of CD154 [32]. A, representative flow cytometric analysis of cells stimulated with various antigens. Restimulation with PMA/Ionomycin was used as a positive control. B, Frequencies of antigen-specific cells obtained from draining lymph nodes 5 weeks after mCOL7c-GST immunization are shown (n = 6). Similar frequencies were determined at week 3 after immunization (data not shown). Data are representative for more than three independent experiments.