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. 2013 Dec 26;8(12):e83631. doi: 10.1371/journal.pone.0083631

Figure 5. T cells, plasma cells and antibodies specific for immunizing antigens.

Figure 5

Mice were immunized with either mCOL7c-GST or mCOL7c-HIS. A, CD4 T cells from draining lymph nodes specific for mCOL7c-HIS, mCOL7c-GST and GST were determined as CD154+ described in Fig. 1. Average background values of negative controls (unstimulated and OVA stimulated cells) were subtracted in all samples. Examples show T cell numbers at week 3 after immunization (n = 6). B, In the same experiment, the numbers of plasma cells specific for mCOL7c were determined as described in Fig. 2 (n = 6). C, mCOL7c-specific serum antibodies 8 weeks after immunization with mCOL7c-GST or mCOL7c-HIS determined by ELISA (n = 3–4). Data are representative for two independent experiments.