| pSARM-GagGFP |
GagGFP F-EagI |
5'-TGCGTCTCAACCTCGGCCG |
|
GagGFP R-AgeI |
5'-CGACCGGTCCCGCACCTTCATACTGTGTTGGAGG |
|
GFP F-AgeI |
5'-TGCCTCCTCCAACACAGTATTGAGTGCGGGACCGGT |
|
GFP R-PspXI + SA |
5'-ACTCTCCTCGAGGAGAGAATCACTTGTACAGCTCGTC CATGCCGA |
| PSARM-GagGFP-M100A |
Kozak F-EagI ultramer |
5'-CAACCTCGGCCGGCGATTAAAAGTGAAAGTAAACTCTCTTGGCCGCCGCGGGAACCTGCCGCGTTGGACCTGAAAGT AAGTGTTGGCCGCCACCATGGGACAGGAGCTGTCACAGCATGAGAGGTATGTGGAGCAGCTGAAGCAG |
|
Kozak R ultramer |
5'-GAAAGCAATCTCCCACTCTTCTCCAGCGCTTAATGTCGATGGTTCCCTCTTGGGGAAACCAAGGACAGGTGTCTTTT ACGAAATCAAAAAATTTCAGAAGGTCGGCGTATTTGACCTTCACTCCCCTGGTTTTAAGGGCCTGCTTCAGCTGCTCCACATA |
|
M100A F ultramer |
5'-GCTGGAGAAGAGTGGGAGATTGCTTTCAGGACTACTATAACACATTTGGCCCCGAAAAAGTTCCCGTTACTGCATTT AGCTACTGGAATCTCATTAAAGAGCTGATTGATAAAAAGGAAGTGAACCCACAGGTCGCTGCCGCAGTCGCGCAGACTGAG |
|
M100A R-SbfI ultramer |
5'-CTTTGTCCTGCAGGGAAGAAGACTTGGCTCCTTCGTCGTCACTATCCAGAGAAATAAGGTCCAAGTCGGGATTCTGT GATGTCTTCGTCAGGTCCGTCTGACTATTGCTTTTAAGAATTTCCTCAGTCTGCGCGACTGCGG |
| pSARM-GagGFP-R55W |
R55W F |
5'-GGAACCATCGACATTAAGTGGTGGAGAAGAGTGGGAGA |
|
R55W R |
5'-TCTCCCACTCTTCTCCACCACTTAATGTCGATGGTTCC |
| pSARM-GagGFP-K16A/K20A |
K16A/K20A F |
5'-CATGAGAGGTATGTGGAGCAGCTGGCGCAGGCCCTTGCGACCAGGGGAGTGAAGGTCAAATAC |
|
K16A/K20A R |
5'-GTATTTGACCTTCACTCCCCTGGTCGCAAGGGCCTGCGCCAGCTGCTCCACATACCTCTCATG |
| pSARM-GagGFP-R22A |
R22A F |
5'-GCCCTTAAAACCGCGGGAGTGAAGGTC |
|
R22A R |
5'-GACCTTCACTCCCGCGGTTTTAAGGGC |
