Table 4. Evaluation of the TRF immunoassays using Syphilis Qualification Panel (QSS701).

		TRF immunoassaysc
Member ID#a	Syphilis reactivityb	10 min incubation	1 h incubation
1	R	125.6 (+)	88.8 (+)
2	R	81.0 (+)	88.3 (+)
3	R	83.3 (+)	91.3 (+)
4	R	49.6 (+)	68.6 (+)
5	R	93.7 (+)	69.3 (+)
6	NR	0.3 (−)	0.3 (−)

^a Members of the panel are manufactured from human serum or plasma, as provided by the panel supplier. Five members are formulated with various reactivities of Syphilis. Non-reactive member was formulated from Syphilis non-reactive pools.

^b Results were provided by the panel supplier. R, reactive; NR, non-reactive.

^c Values indicate S/Co ratios obtained in this study, using the in-house TRF immunoassays with 10 min and 1 h incubation times. Samples with S/Co values <1.0 are designated as negative (−) and those with values ≥1.0 are designated as positive (+).