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. 2013 Dec 26;8(12):e83943. doi: 10.1371/journal.pone.0083943

Figure 5. Knockdown of CLCA4 induces EMT.

Figure 5

HMLE cells were lentivirally transduced with shRNAs GipzH4A, GipzH4B and GipzH4C. GipzNC contained a non-silencing control. A, transcriptional profile of CLCA4 and EMT markers. Values were normalized to HMLE parent. P<0.01 for each pairwise comparison between GipzNC and GipzH4A or GipzH4C. B, immunoblot of whole cell lysates probed for EMT marker proteins. Band intensities in each lane were normalized to the beta actin value. C, CLCA2 downregulation in CLCA4-knockdown cells. Values normalized to HMLE parent. P<0.001.