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. 2013 Dec 26;8(12):e83905. doi: 10.1371/journal.pone.0083905

Figure 2. Combination of YB-AHM plus Len enhanced cytotoxicity against MM cells in BMMCs from MM patients.

Figure 2

(A) BMMCs from a MM patient (no. 3) were pretreated with or without Len (3 µM) for 24 hours, and incubated in the presence of AHM or YB-AHM (0.1 µg/mL) for 24 hours. Thereafter, the cells were stained with control PE-labeled mouse IgG or PE-labeled CD38 mAb, and analyzed by flow cytometry. MM cell populations were indicated within squares according to side scatter (SS) and CD38 expression profiles. (B) Cytotoxic activity was estimated by % increase of dead cells from the baseline of CD38++ MM cell fractions in BMMCs from MM patients (n = 10). Data presented are mean ±SD (**, p<0.01). (C) BMMCs from a MM patient (no. 5) were treated with or without Len (3 µM) and YB-AHM (0.1 µg/mL) as above. Cytospun preparations were stained with a Wright-Giemsa solution, and examined under a microscope (original magnification, x 1000).