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. 2013 Dec 26;9(12):e1003823. doi: 10.1371/journal.ppat.1003823

Figure 5. SalmonE and mAb 73.18 recognize CST1 (TGME49_064660).

Figure 5

(A) Immunoblot of mAb SalmonE immunoprecipitated material (ME49), WT lysate (Pru) and Δcst1 lysate probed with mAb SalmonE (green) and mAb 73.18 (red). This immunoblot demonstrated that mAb SalmonE and mAb 73.18 bind to the same major species which is not detectable in Δcst1 parasites. (B) IFA of in vitro cysts probed with CST1 specific monoclonal antibody 73.18 (red) and DBA lectin (green). The presence of full-length CST1 is required for both mAb 73.18 and DBA cyst wall staining. Bar, 10 µm.