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. 2013 Dec 26;9(12):e1003825. doi: 10.1371/journal.ppat.1003825

Figure 5. The pUL21a cyclin-binding domain is essential for HCMV to prevent cellular DNA synthesis.

Figure 5

MRC-5 cells were infected with indicated viruses in the presence of viral DNA replication inhibitor phosphonoacetic acid (PAA). Cells were collected at 24 and 48 hpi, fixed, and co-stained with propidium iodide (PI) and antibody to viral protein pUL44. Cell cycle profiles were analyzed by flow cytometry. (A) The PI and pUL44 staining profiles of representative mock- and wildtype virus- infected cells at 48 hpi. Also shown is gating used to separate pUL44-positive (infected) and pUL44-negative (uninfected) cells. (B) Both 24 and 48 hpi DNA content profiles of mock-infected cells or pUL44-positive, virus-infected cells. (C) Percentage of pUL44-positive, virus-infected cells in G1, S, or G2/M phase at 48 hpi based on DNA content. p values were based on the numbers of S phase cells and determined using the student's t test. *, p value <0.05; n.s. (not significant), p value >0.05. Shown are data from two independent experiments.