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. 2013 Dec 26;9(12):e1003825. doi: 10.1371/journal.ppat.1003825

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© 2013 Caffarelli et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) MRC-5 cells were infected with indicated viruses at an MOI of 0.01 TCID₅₀/cell and production of cell free virus was determined by TCID₅₀ assay at indicated time points. (B) MRC-5 cells were infected with indicated viruses at an MOI of 3, and cell lysates were collected at 24 and 48 hpi and analyzed by immunoblotting. * indicates nonspecific cross-reacting bands. (C) MRC-5 cells were treated with siRNA against cyclin A (siCyclin A) or luciferase control (siCont), and then infected with indicated virus at an MOI of 3. At 96 hpi, production of cell free virus was determined by TCID₅₀ assay, and (D) cell lysates were analyzed by immunoblotting.