Table 1. Primers used in this study.

Purpose	Primer sequencea	Construct name
Overlapping PCR primers to create UL21a RxL-AxA mutation	5′cgagctcgtgcgagggctttccaaaatc3′ 5′gattttggaaagccctcgcacgagctcg3′	Non applicable
Overlapping primers to create template for amplifying ChUL21a	5′atgggaggcagtcccgaccccgatctctcggtggccaccgagggtgtgaggcccgtggtccgcgcggacctgttccgagcccgccggcccctgc3′ 5′cctgaaggtgctggtgatgctgatggtagttctcaaaaagccggcgccgggcccgcggagcatagaaagccagacggcgcaggggccggcgggct3′ 5′tcaccagcaccttcaggtcccgccgccgattcatcggatcgtcgctgtgcccggaggggacgaggaagcgataccgatggacctgccgagggaga3′ 5′tgggcggaacgtcgtccaacagcagcaccagcgggttgggcagcgggcggtcgggcggtatatcggcggcgacctggatctccctcggcaggtcc3′ 5′ttaaaaatgttcccagttctcttcgcgtatcacggggtactcgcggggcactcggaaaggagcgaagccgggcggcatgggcggaacgtcgtcc3′	Non applicable
PCR primers to clone UL21a into pLKO vector	5′gtcgaccgagatgggaggtagccctgttcc3′ 5′ggaattcttaaaactggtcccaatgttctt3′	pLKO-UL21a pLKO-UL21a RxL-AxA
PCR primers to clone RhUL21a into pLKO vector	5′gtcgaccgagatgggaggcagcaccga3′ 5′ggaattcttaagcgtgtgcttcttcat3′	pLKO-RhUL21a
PCR primers to clone ChUL21a into pLKO vector	5′gggtcgacatgggaggcagtcccgac3′ 5′gggaattcttaaaaatgttcccagttctc3′	pLKO-ChUL21a
PCR primers to clone 3xFlag-Cyclin A into pLKO vector	5′gcgtcgacatggactacaaagaccatgacggtgatttaaagatcatgatatcgattacaaggatgacgatgacaagttgggcaactctgcgcc3′ 5′gcgaattcttacagatttagtgtctctggtggg3′	pLKO-3xFlag-Cyclin A pLKO-3xFlag-Cyclin A ΔD-box
PCR primers to clone HA-Ubiquitin into pLKO vector	5′gcgctagcatgtacccatacgacgtccca3′ 5′gcgaattctcacccacctcttagtctt3′	pLKO-HA-Ubiquitin
Primer pairs to quantify cyclin A transcript levels by RT-qPCR	5′gcatgtcaccgttcctcctt3′ 5′cagggcatcttcacgctctat3′	Non applicable
Primer pairs to quantify GAPDH transcript levels by RT-qPCR	5′ctgttgctgtagccaaattcgt3′ 5′acccactcctccacctttgac3′	Non applicable

^a Restriction sites are underlined; base pair changes to introduce mutations are underlined and in italics.