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. 2014 Jan;184(1):200–213. doi: 10.1016/j.ajpath.2013.09.010

Figure 1.

Figure 1

TAT-mediated transport facilitates uptake of the PKCδ inhibitory peptide throughout the pulmonary endothelium and peripheral lung parenchyma. Rat lungs were gravity-fixed 30 minutes after IT administration of a fluorescent TMR-tagged TAT-conjugated PKCδ inhibitory peptide. Representative lung tissue sections are shown (n = 3 animals). A: TMR fluorescence (red) reveals largely homogeneous distribution of the peptide throughout the peripheral lung parenchyma, in the endothelium of small veins and arterioles (arrows), and in alveolar wall cells (inset). B: Robust TMR fluorescence (red) indicates uptake of the peptide in large vessel endothelium (arrows) and surrounding parenchyma. C: Cropped view of TMR fluorescence (red) in the capillary of an alveolar septum (arrows), indicating uptake of the peptide at the level of capillary endothelium. Original magnification: ×100 (A and B); ×400 (C).