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. 2014 Jan;184(1):200–213. doi: 10.1016/j.ajpath.2013.09.010

Figure 6.

Figure 6

Neutrophil binding to IL-1β–activated PMVECs is PKCδ dependent. PMVEC monolayers were treated overnight with buffer or 10 U/mL IL-1β ± 0.5 to 10 μmol/L PKCδ inhibitor. Calcein-loaded neutrophils (2 × 105) were incubated with PMVECs for 30 minutes at 37°C. Neutrophil binding was calculated from a standard curve prepared from calcein-loaded neutrophils. Data are expressed as means ± SEM from one representative experiment, performed in triplicate, from four different neutrophil donors. ∗∗P < 0.01 IL-1β versus IL-1β+PKCδ inhibitor (1 to 10 μmol/L); ∗∗∗P < 0.001 buffer versus IL-1β.