Table 1.

Primer Sequences

Primer name	Sequence	Use
LF	5′-AACCTCCCCAGGCTCAGTAA-3′	LHA forward-screening primer
LR	5′-AAATCCTCCTCGTTTTTGGA-3′	LHA reverse-screening primer
RF	5′-CAGGTTCAGGGGGAGGTGTG-3′	RHA forward-screening primer
RR	5′-ATGTCTGGCTTCCACCTCACTAAC-3′	RHA reverse-screening primer
CF	5′-CTTTACACAGAGGTGCCCAAT-3′	Forward primer to genotype PALB2-null cells
CR	5′-CTCCCAGGTTCAAGCGACT-3′	Reverse primer to genotype PALB2-null cells
RT-PCR
Forward	5′-AGTGCCATGTTTTGGGAAAG-3′	RT-PCR to confirm PALB2 exon 8 deletion
Reverse	5′-TCCATCTTCTGCAAACGTCA-3′	RT-PCR to confirm PALB2 exon 8 deletion

LHA, left homology arm; RHA, right homology arm.