Figure 3.

NAC1 is both required and sufficient for FOXQ1 expression. A: Western blot analysis demonstrates a decrease in FOXQ1 protein levels 48 hours after NAC1 knockdown in both SKOV3 and HeLa cells. GAPDH was used as the loading control. B: Western blot analysis shows an increase in FOXQ1 protein levels 48 hours after ectopic NAC1 expression in NACC1^−/− mouse ovarian surface epithelium. GAPDH was used as a loading control. C: qPCR demonstrates that FOXQ1 expression was induced 24 hours after transfection of a NAC1 expression construct into NACC1^−/− mouse fibroblasts. D: Expression of N130, the truncated form of NAC1 comprising only the BTB domain, down-regulates FOXQ1 transcript level in HeLa cells compared with noninduced cells at different time points.