Figure 4.

NAC1 regulates FOXQ1 promoter activity. HeLa or SKOV3 cells were first transfected with two independent NAC1 siRNAs to knock down NAC1 expression. One day later, the pGL3-FOXQ1 (black bars) promoter construct or pGL3 control plasmid (white bars) was transfected into the cells. Luciferase activity was measure 24 hours after transfection of the promoter construct. FOXQ1 promoter activity in NAC1 siRNA-treated cells is significantly lower than in the control siRNA-treated cells. ^∗∗P < 0.01.