Figure 6.

Expression of proteins involved in centrosomal abnormalities. A: Western blots (n = 3) show that levels of p53 and p21 are not affected in cystic cholangiocytes, but expression of Cdk2 and cyclin E is increased. Cdc25A phosphatase, the upstream activator of the Cdk2–cyclin E complex, is also up-regulated. B: Overexpression of Cdk2, cyclin E, and Cdc25A was confirmed by confocal microscopy in cholangiocytes of rodents and patients with polycystic liver diseases. Proteins of interest stain green; nuclei stain blue. Features marked by an asterisk are shown without nuclear staining in the corresponding inset. Data are expressed as means ± SEM. ^∗P < 0.05. Original magnification, ×63. C, control.