Figure 8.

A: Depletion of Cdc25A by shRNA or by miR-15a precursor decrease number of centrosome per cell and fraction of cholangiocytes with multiple centrosomes. B: Ciliogenesis was examined by confocal microscopy in PCK-Cdc25A-sh cholangiocytes. C: Cyst growth was evaluated by light microscopy in PCK-miR-15a-pre cholangiocytes. Centrosomes were stained with γ-tubulin (green) and cilia with α-tubulin (red); nuclei were counterstained with DAPI (blue). Data are expressed as means ± SEM. n = 50 cholangiocytes (A and B) and n = 25 cysts (C) for each condition. ^∗P < 0.05. Original magnification: ×100 (A and B); ×4 (C). EV, empty vector.