Table 2.

Limits of Mutation Detection for Three Variants

Sample	Variant reads at each dilution
	FLT3-ITD	NPM1	DNMT3A
Undiluted∗	120	78	85
1:2	80	59	67
1:4	27	21	19
1:8	25	16	18
1:16	3	6	ND

Mutations: FLT3-ITD c.1804_1805ins24bp, NPM1 c.860_863dup, DNMT3A c.1627G>T (p.G543C).

ND, not detected.

^∗45% tumor cellularity undiluted; acute myeloid leukemia (AML) sample HEME49 used for mixing study.