Figure 5.

Sensitivity and false positive calls made using only filtered reads compared with using all reads. Filtered reads included only those with a mapping quality >20, a minimum base quality of 20 for all bases, and no more than four discrepancies. A: Sensitivity for minor gold standard SNVs across all four programs after filtering of low-quality and multiple-discrepancy reads. Error bars show the 95% binomial CI for each point estimate. B: False positive SNV calls (mean per sample) using high-quality reads compared with all reads. Error bars show the SD across all samples at each mix proportion (n = 4).