Fig. 3.

The global 235-239-240 suppressor motif rescues many common p53 cancer mutants in mammalian reporter gene assays for the p21 promoter. p53 cancer mutants with (black bars) and without (gray bars) suppressor amino acids were evaluated as described for Fig. 2, except that a reporter plasmid with the intact p21 promoter (2.3 kb) was used. The transcriptional activity of all p53 cancer mutants with suppressor amino acids was significantly better when compared with the results of Fig. 2, suggesting that p53 coactivators recruited to the p21 promoter can further enhance the effect of suppressor amino acids.