Figure 3. In vitro.
characterization of LbL films. (A) SEM image showing the regular woven pattern of Laponite® containing film coated on Tegaderm® used for in vitro testing. Scale bar = 100 μm, inset scale bar = 10 μm. (B) SEM of uncoated Tegaderm® substrate. Similar scales as in A. (C) Plot of flow cytometry data for NIH-3T3 cells stably expressing GFP exposed to LbL film coated Tegaderm for 3(■) 5(
) and 7 (
) days. Data is shown as relative mean cell fluorescence normalized to cells treated with siControl containing films of the same architecture. (D) Cell viability of NIH-3T3 cells exposed to LbL film coated Tegaderm as compared to cells exposed to uncoated Tegaderm®, measured using AlamarBlue® metabolic assay.