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siRNA released from LbL assembly continues to transfect cells and remains active over 1 week period in vitro. (A-C) Uptake of FITC-labeled siRNA by NIH-3T3 cells at 3 (A), 5 (B), and 7 (C) days exposure to LbL films containing labeled siRNA. Cells were seen to become more diffusely fluorescent over the one week period. Scale bar = 10 μm. (D) Average fluorescent intensity of cells.
