Figure 2.

A CD4⁺ T-cell niche for long-lived plasma cells in rituximab-treated spleens. (A) Confocal microscopy of sections of rituximab-treated spleen stained with anti-CD3 (blue), anti-kappa/lambda light chains (green), and Hoechst (cyan). Plasma cells are located adjacent to the T-cell zone and in red pulp. Red squares mark plasma cells. (B,C) Confocal microscopy of sections of rituximab-treated spleen stained with anti-kappa/lambda light chains (green), anti-CD3 (red), and anti-CD8 (blue) (B), or anti-CD3 (red) and anti-CD4 (blue) (C). (D) Sections of rituximab-treated spleen stained with anti-kappa/lambda light chains (green) and anti-CD4 (red). Data are representative of three spleen samples. Scale bars: gray 100 μm, blue 5 μm.