Table 1. Determination of β-AR density by radioligand binding.

	15 min		28 days
Treatment	Bmax, fmol·mg−1 protein	KD, pM	Bmax, fmol·mg−1 protein	KD, pM
Ctrl	286.8 ± 88.02	107.9 ± 43.67	286.8 ± 88.02	107.9 ± 43.67
NTX	109.2 ± 9.72*	193.6 ± 20.66	109.2 ± 9.72*	193.6 ± 20.66
Salbutamol	256.5 ± 29.24†	228.8 ± 33.07	97.0 ± 23.02	225.4 ± 41.79
Alprenolol	299.5 ± 12.19†	453.6 ± 86.33	179.2 ± 53.05	290.9 ± 55.07
Carvedilol	86.3 ± 19.42	565.2 ± 192.8†	904.1 ± 43.46†	1444.0 ± 202.0†
Nadolol	181.9 ± 48.28	695.1 ± 286.3†	785.5 ± 154.8†	1591.6 ± 335.0†

β-AR density was measured in membranes prepared from lung tissue using the β-AR radioligand (−)3-[¹²⁵I]-iodocyanopindolol in increasing concentrations (5-7,500 pM). Samples were run in triplicate and values are mean ± SEM of n = 3-5 animals in each group. Please note, the 15-min and 28-day time point refers to duration of drug treatment. All mice were killed at the same age; and thus for vehicle-treated groups (Ctrl and NTX), the groups were identical and the results were pooled.

^*P < 0.05 compared to Ctrl.

^†P < 0.05 compared to NTX (Student's t test).