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Effect of suppression of PTPα on the recruitment of focal adhesion proteins to FAK. FAK was immunoprecipitated (IP) from serum-deprived 10A.B2 or 10A.B2-shPTPα cells plated on fibronectin (25 μg/ml), incubated or not incubated with AP1510 (1 μm) for 30 min, and lysed. Proteins were separated by SDS-PAGE and immunoblotted using anti-vinculin, anti-paxillin, anti-GRB7, anti-HA, or anti-FAK antibodies. Lysates were probed for PTPα expression.
