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. 2013 Nov 11;288(52):36926–36935. doi: 10.1074/jbc.M113.527564

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Effects of suppressing PTPα on GRB7-ErbB2 signaling in 10A.B2 cells. A, association of GRB7 with ErbB2. Serum-deprived 10A.B2 and 10A.B2-shPTPα cells were treated with AP1510 (1 μm) for the indicated times. HA-ErbB2 was immunoprecipitated (IP) from 100 μg of cell lysate using anti-HA and immunoblotted using anti-GRB7 antibody. ErbB2 was detected using anti-HA antibody, and lysates were probed for actin and PTPα for loading and PTPα expression. B, sequence of GRB7 SH2-domain inhibitor peptide G7–18NATE. C, the effects of GRB7 inhibitor peptide on ErbB2-induced migration in 10A.B2-shPTPα cells. 10A.B2 and 10A.B2-shPTPα cells were seeded in transwell migration chambers in the absence (−) or presence (+) of 1 μm AP1510, 10 μm G7–18NATE-Penetratin (WFEGYDNTFPC-RQIKIWFQNRRMKWKK), or 10 μm penetratin peptide (RQIKIWFQNRRMKWKK), incubated for 48 h, and then migration was quantitated as described under “Experimental Procedures.”