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. 2013 Nov 20;288(52):36983–36993. doi: 10.1074/jbc.M113.500264

FIGURE 7.

FIGURE 7.

Accelerated apoptosis of M1 cells ectopically expressing cMybT486A. A, M1 cell lines ectopically expressing either wild-type (cMybWT) or T486A mutant of c-Myb (cMybT486A) treated with anisomycin (5 μg/ml) for indicated time and evaluated for viability by trypan blue exclusion assay. Data (percentages of dead cells in cell cultures) are shown as averages ± S.E. (error bars) from three independent experiments. *, p < 0.05. The inset shows expression of c-Myb and tubulin in M1/cMybWT and M1/cMybT486A cells. B, cleavage of PARP protein (cl-PARP) in cells treated with anisomycin evaluated by immunoblotting. The relative intensities of cl-PARP in M1/cMybWT and M1/cMybT486A normalized to levels of tubulin (loading control) are shown below immunoblot. C, apoptotic DNA ladder in M1 cells ectopically expressing cMybWT and cMybT486A untreated (left panel) or treated with anisomycin for 6 h (right panel). D, expression of Bcl-2, Bcl-xL, Mcl-1, and actin in M1/cMybWT and M1/cMybT486A cells untreated (left panel) or treated with anisomycin for 6 h (right panel). E, bar graph presents intensities of Bcl-2, Bcl-xL, and Mcl-1 in anisomycin-treated M1 cells normalized to actin.