FIGURE 6.

Generation of transgenic mice containing haplotype I and II of the hAT₁R gene: Transgenic mice were generated using 166-kb BAC plasmid DNA containing hAT₁R gene by recombineering as described under “Experimental Procedures.” A, diagrammatic presentation of the 5′-UTR, coding region, and 3′-UTR of hAT₁R gene of BAC construct in 7.4 kb of pBeloBAC11 vector. B, restriction digestion of BAC containing hAT₁R gene with restriction enzyme SpeI followed by gel electrophoresis was performed to determine authenticity of the BAC. Lane 1 shows the molecular size marker of the 1-kb ladder DNA; lanes 2 and 3 show DNA fragments obtained from the restriction digestion of haplotypes I and II, respectively. C, location of primers designed from different regions of the BAC plasmid for the characterization of hAT₁R gene.