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Actin-activated ATPase activity of phosphorylated MYO3A 2IQ ΔK. A, comparison of phosphorylated MYO3A 2IQ ΔK (treated with MYO3A kinase for 60 min) and control-treated MYO3A 2IQ ΔK demonstrates phosphorylation reduces motor ATPase activity. B, antiphosphothreonine Western blot demonstrates MYO3A 2IQ ΔK phosphorylation compared with control treated. Data represent averages from two separate experiments (error bars represent mean ± S.E.).
