TABLE 2.

Kinase activity of MYO3A constructs

Construct	Kinase activitya	Moles of 32P/mol of MYO3Ab	One-half timec
	min−1		min
WT MYO3A 2IQ	0.173 ± 0.004	1.28 ± 0.35	2.93 ± 0.17
MtDd MYO3A 2IQ	0.113 ± 0.001	1.50 ± 0.22	3.44 ± 0.48
T184E MYO3A 2IQ	0.173 ± 0.030	1.28 ± 0.47	2.85 ± 0.16
T184A MYO3A 2IQ	0.006 ± 0.001	0.33 ± 0.22	12.62 ± 1.15
T178D MYO3A 2IQ	0.094 ± 0.007	0.82 ± 0.07	3.32 ± 0.68
T178A MYO3A 2IQ	0.034 ± 0.001	0.58 ± 0.22	4.79 ± 0.23
MYO3A kinase	0.026 ± 0.002	0.54 ± 0.13	5.22 ± 0.75

^a Data were determined from the initial velocity of the kinase reaction with [³²P]ATP.

^b Data were determined from the 30- or 60-min time point of the kinase reaction with [³²P]ATP.

^c Data were determined from the exponential fits of the phosphorylation time courses (Figs. 3B and 4B).