TABLE 3.
Actin-activated ATPase activity of MYO3A 2IQ constructs
Phos, phosphorylated; UnPhos, unphosphorylated.
| Construct | V0 | kcat | KATPase | kcat/KATPase |
|---|---|---|---|---|
| s−1 | s−1 | μm | μm−1·s−1 | |
| WT MYO3A 2IQ | 0.15 ± 0.02 | 1.1 ± 0.1 | 20.5 ± 5.1 | 0.054 ± 0.014 |
| T184E MYO3A 2IQ | 0.11 ± 0.02 | 1.6 ± 0.1 | 46.7 ± 5.5 | 0.035 ± 0.005 |
| T184A MYO3A 2IQ | 0.11 ± 0.04 | 1.0 ± 0.1 | 55.3 ± 18.2 | 0.018 ± 0.006 |
| T178D MYO3A 2IQ | 0.13 ± 0.03 | 1.3 ± 0.1 | 28.1 ± 6.8 | 0.046 ± 0.012 |
| T178A MYO3A 2IQ | 0.10 ± 0.03 | 0.7 ± 0.1 | 22.6 ± 7.4 | 0.031 ± 0.011 |
| MYO3A 2IQ ΔK UnPhos | 0.08 ± 0.01 | 1.8 ± 0.1 | 4.0 ± 0.9 | 0.450 ± 0.110 |
| MYO3A 2IQ ΔK Phos | 0.11 ± 0.01 | 1.0 ± 0.1 | 20.4 ± 3.9 | 0.049 ± 0.011 |