FIGURE 2.
Gating strategies for the flow cytometry. A, for all analyses in this study, cells were gated to exclude the smallest particles and clumped cells. B, untransfected cells were excluded from the analysis using untransfected cells to reference the gate boundary. C, SYTOX Red Dead was gated based on permeability into cells. D, PuLSA gating strategy of Httex1TC9-Cerulean was described previously (19). E, FlAsH reactivity of cells expressing Httex1TC9-Cerulean was gated into PFR population and high FlAsH-reactive population as described previously (19).