FIGURE 9.
hsp40 and hsp70 influence on molecular partitioning of Httex1 as monomers, oligomers, and inclusions. The proportion of Httex1-Emerald molecules in Neuro2a cell lysate is shown as defined by gel filtration (which can quantitate monomers and oligomers) and SV analysis in 2 m sucrose at 3,000 rpm (which can quantitate inclusions). Cells were transfected with equal DNA quanta of Httex1-Emerald and hsp40 and hsp70, buffered with the GFPinv construct under the same conditions as for the flow cytometry data in Figs. 3 and 4. Total abundance of Htt based on fluorescence yield in lysate (n = 3, mean ± S.D. shown). For ANOVA, individual pairwise comparisons against Htt alone as control are shown for p < 0.05. Proportions of monomers, oligomers, and inclusions (n = 3, mean ± S.D. shown). For ANOVA, individual pairwise comparisons against Htt alone as control are shown for p < 0.05.