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Effect of spin-labeled EF-hand domain mutants on line widths of acyl chain 1H resonances in 4 mmd54-DMPC/POPA (1:1) SUVs. Spin-labeled EF-hand domain C148S was specifically labeled on Cys-188, (CH2)n (○), and ω-CH3 (Δ); spin-labeled EF-hand domain C188S was specifically labeled on Cys-148, (CH2)n (●), and ω-CH3 (▴). Control line widths for the same SUVs mixed with unlabeled protein are also shown: (CH2)n (×); ω-CH3 (+).
