TABLE 2.

Apparent dissociation constants for rEF with mutations of basic residues binding to POPC/DOPA (1:1) SUVs in 20 mm Tris, 0.1 mm EGTA, pH 7.5

EF constructs	Apparent KDa	KD (mutant)/KD (WT)
	mm
WT	0.12 ± 0.02	1.0
R150A/K151A	0.51 ± 0.04	4.3
R150A/K151A/K154A	0.41 ± 0.07	3.4
R150A/K151A/K154A/K156A	0.44 ± 0.06	3.7
K163A	0.20 ± 0.03	1.7
K170A	0.27 ± 0.08	2.3
R186A	0.23 ± 0.06	1.9
R182A/K183A/R186A	0.35 ± 0.02	2.9

^a This is the total concentration of phospholipids present for half of maximum binding. Errors were determined from the hyperbolic fit to the data.